Doctoral Thesis

 

 

• Optimization of collagen type I scaffold production and study of the effect of microstructure on artificial oral mucosa development.  Edward Suesca Quintero. 2014. Doctoral Program in Pharmaceuticals Sciences, Faculty of Sciences , National University of Colombia.

 

• Performance of artificial tissue as grafts on rabbit oral mucosa wounds and in vitro evaluation of its chemotactic role.Lady Espinosa Aroca. 2011. Doctoral Program in Biotechnology, Faculty of Sciences , National University of Colombia.  Merit Distinction.

 

 

 

MSc Thesis

 

• Evaluation of biocompatibility and efficiency as artificial tissue substitute of type I collagen scaffolds associated with  natural extracts derived of Calendula officinalis in a lagomorph animal model of skin wound. Diana Milena Millán Cortés. 2014. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.

 

• Secretion of growth factors and cytokines during the production of artificial tissue under two different oxygen concentrations in a Spinner bioreactor. Diana Isabel Nieto Patarroyo. 2014. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.

 

• Standardization of a purification method of type II collagen and preparation of cartilage tissue engineering scaffolds.  María Elisa Soto Herrera. 2014. Master Program in Chemical Engineering. Departament of Chemical Engineering.  Faculty of Engineering, National University of Colombia.  Merit Distinction.

 

• Optimization of type I collagen scaffolds as a controlled delivery system for a natural extract derived of marigold (Calendula officinalis).  Ronald Andrés Jiménez Cruz. 2013. Master Program in Pharmaceutical Sciences.  Faculty of Sciences, National University of Colombia.

 

• Evaluation of the repair process in rabbits oral mucosa wounds grafted with autologous artificial connective tissue prepared ​with unidirectional and multidirectional collagen scaffolds. Andrea Escalante. 2013. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.

 

• Development of type I collagen scaffolds associated with hemoglobin isolated from human peripheral blood.  Julio César Ríos Camacho. 2012.  Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.

 

• Evaluation of the growth factors expression involved in oral mucosa wounds in artificial connective tissue.  José Manuel González. 2012. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.  Merit Distinction.

 

• Evaluation of the CO2 transfer to culture medium in a Spinner-like bioreactor for growing fibroblasts. Sergio Casadiego. 2011. Master Program in Pharmaceutical Sciences.  Faculty of Sciences, National University of Colombia.  Merit Distinction.

 

• Characterization of a Spinner-like bioreactor mixing.  Luz Angélica Castro. 2008. Master Program in Chemical Engineering. Departament of Chemical Engineering.  Faculty of Engineering, National University of Colombia. 

 

• Modelling glucose and oxygen diffusion in fibroblast seeded collagen scaffolds. Paula Pulido. 2008. Master Program in Chemical Engineering. Departament of Chemical Engineering.  Faculty of Engineering, National University of Colombia. 

 

• Evaluation of growth of fibroblasts seeded into collagen scaffolds in a spinner bioreactor. Juan Felipe Díaz. 2008. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia. 

 

• Evaluation of Aggregation Substance (AS) role in Enterococcus faecalis invasión to HUVEC cultures. 2008.  Andrea Victoria Castillo. 2008. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia. 

 

• Determining of chromosomal stability in fibroblasts isolated from rabbit oral connective tissue .Diana Patricia Martínez Hernandez.  2008.  Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia. 

 

• Effect of cytoskeleton polimerization inhibitors in Enterococcus faecalis invasión to HUVEC cultures.  Orfa Ines Contreras. 2005. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia. 

 

• Development of a model based on Caco-2 cell monolayers with application in drug absorption studies. Rocío Castillo. 2004.  Master Program in Sciences - Pharmacology.  Faculty of Sciences, National University of Colombia.  Merit Distinction.

 

• Evaluation of vancomycin effect on enterococcal nosocomial isolates invasion to HUVEC cultures.  Sandra Milena Cháves. 2003. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia. 

 

• Identification of specific inflammatory genes expressed in macrophages by cDNA microarrays. Vera Ripoll. 2003. Master Program in Sciences - Microbiology. Faculty of Sciences, National University of Colombia. Laurate Distinction.

 

• Standardization of a method to isolate and culture oral fibroblasts. Samir Alejandro Bello Melo. 2002. Master Program in Sciences - Microbiology. Faculty of Sciences , National University of Colombia.  Merit Distinction.

​​

Undergraduate Thesis

• Evaluation of skin wound closure treated with type I collagen scaffolds associated with a hydroglycolic natural extracts derived of Marigold flowers in a murine animal model pilot trial. Hector Alveiro Peña Vacca.  2013.  Pharmaceutical Chemistry Program.  National University of Colombia. Distinction.

 

• Evaluation of type I collagen scaffolds as a delivery system of a natural extract of  Marigold (Calendula officinales). Gabriel Jaime Zea.  2011.  Pharmaceutical Chemistry Program.  National University of Colombia. Distinction.

 

• Development of a detection technique for Vascular Endothelial Growth Factor (VEGF) present in culture medium of autologous artificial tissue in an optical biosensor of  surface plasmon resonance.  Yenith Alexandra Cuellar Sáenz, Jenny Vanessa Valbuena Pérez.  2012.  Pharmaceutical Chemistry Program.  National University of Colombia.

 

• Adaptation of an extraction technique for hyaluronic acid from bovine vitreous humor.  Natalia Bucheli Ibarra, Martha Duque, Adriana Iglesias, Sandra Jimenez, Olga Londoño. 2008.  Medicine Programe. El Bosque University - Colombian School of Medicine.  

 

• Determining microstructure of type I collagen scaffolds for use in tissue engineering. Nathalia Barbosa Acevedo, Daniel Arturo Diaz Cardona, Carlos Andrés Téllez Olaya, Mauricio Téllez Olaya.  2008.  Medicine Programe. El Bosque University - Colombian School of Medicine.  

 

• Protocol for isolation and preparation of collagen scaffolds isolated from porcine tendons. Julio César Ríos Camacho.  2007.   Medicine Programe. El Bosque University - Colombian School of Medicine. 

 

• Standardization of a method for obtaining type I collagen scaffolds with chondroitin sulphate. Diana Carolina Plata Rodríguez, José Fernando Forero Paipa. 2007. Medicine Programe. El Bosque University - Colombian School of Medicine. 

 

• Study of enzymatic activity of Monocytes Specific Carboxylesterases  ( MSCEs ) in monocytes cultures isolated from healthy and atherosclerotic disease patients: implications for the development of atherosclerosis. Andrea Paola Najar.  2006.  Bacteriology Program.  Universidad Colegio Mayor De Cundinamarca. 

 

• Study of enzymatic activity of MSE ( Monocyte Specific esterases ) and CHS ( cholesterol ester hydrolase ) in monocyte, macrophage and foam cell lineage.  Adrian Gabriel Sandoval.  2005.  Chemistry Program. National University of Colombia.

 

• Construction and commissioning of a bioreactor for the fibroblasts growth in type I collagen scaffolds to bank level . Juan Felipe Diaz, Paula Arévalo.  2005. Chemical Engineering Program.  National University of Colombia.

 

• Role of lipid metabolism of monocyte and macrophage in foam cell formation. Edward Suesca.  2005. Pharmaceutical Chemistry Program.  National University of Colombia.

 

• Standardization of a primary cell culture method of keratinocytes and his co- culture with fibroblasts in type collagen scaffolds.  María Mercedes Posada.  2004.  Pharmaceutical Chemistry Program.  National University of Colombia. 

 

• Manual of cystic fibrosis.  Xiomara Rocío Rodríguez Bersebi, Yaneth Velandia Barrera, María Milagros Villadiego Arrieta.  2003.  Medicine Programe. El Bosque University - Colombian School of Medicine. 

 

• Standardization of in vitro cell culture technique of macrophages obtained from atherosclerotic plaques extracted from hyperlipidemic rabbits. Juan Carlos Porras.  2002. Pharmaceutical Chemistry Program.  National University of Colombia. 

 

• Establishment and characterization  of an endothelial cells primary culture obtained from human umbilical vein.  Francisco Javier Ramírez y Sandra Milena Rivera.  2002.   Pharmaceutical Chemistry Program.  National University of Colombia. 

 

• Standardization of an isolation and characterization method  of Type II Collagen in Cultured Porcine chondrocytes of Fetuses In Vitro.  Ivon Ariza Patiño, Estrella Durán Gutierrez, Mónica Sarmiento Lopez.  2002.   Medicine Programe.  El Bosque University - Colombian School of Medicine. 

 

• Initial healing of grafted areas with oral mucosa analogues in Experimental Animals.  Edna Paola Carvajal Barrera.  2002.  Medicine Programe.  El Bosque University - Colombian School of Medicine. 

 

• Standardization of a method for the analogue of human dermis preparation. Adriana Doncel y Silvia Pérez. 2001.  Pharmaceutical Chemistry Program.  National University of Colombia. 

 

• Immunohistochemical detection of fibronectin and collagen III in oral connective tissue. Lila Margarita Acosta Medina, Eliana Agudelo Ardila, María Ximena Corredor Castillo, Paula Andrea Duarte Alarcón, Liliana del Pilar Yate Forero.  2000.  Dentistry Program.  El Bosque University - Colombian School of Medicine. 

 

 

 

Especialization Research Works

 

• Co- culture of conjunctival epithelial cells and fibroblasts. Girleza Montoya Guacaneme.  2011.   Specialization in Ophthalmology.  Faculty of Medicine.  National University of Colombia. 

 

• Development of a co- culture of cells (goblet and fibroblasts ) isolated from rabbit conjunctiva.  Andrea Carolina Vásquez Roa.  2009. Specialization in Ophthalmology.  Faculty of Medicine.  National University of Colombia. 

 

• Standardization of a technique for in vitro culture of isolated cells in brain gliomas.  Javier Lobato y Oscar Feo.  2004. Specialization in Neurosurgery.  El Bosque University - Colombian School of Medicine.     

 

• Obtention of fibrin scaffolds as extracellular matrix in chondrocytes headphones cell cultures. Hugo Balaguera. 2004.  Specialization in Plastic Surgery.  El Bosque University - Colombian School of Medicine.     

 

 

Internships

• Research area of artificial skin cultures. Camilo Andrés Caicedo Tapia, Nazly Mayerly Cuevas Meléndez, Isabel Rocío Rodríguez Sánchez, Juan Manuel Torres Cañizales, Laura del Pilar Vargas Barrera.  2005. Medicine Programe.  El Bosque University - Colombian School of Medicine. 

 

 

Scientific Initiation Research

• Effect of cytoskeletal polymerization inhibitors, colchicine and cytochalasin D, on the invasion of Enterococcus faecalis in endothelial cells isolated from umbilical cord. Olga Maritza Berrio. 2004.  Young Investigator .  Administrative Department of Science , Technology and Innovation, Colciencias. Molecular Biology Institute.   El Bosque University - Colombian School of Medicine. 

 

• Paracrine effect evaluation of factors secreted by artificial connective tissue in partial thickness wounds. Diana Millan.  2011. Young Investigator.  Administrative Department of Science , Technology and Innovation, Colciencias.